【Objective】 The study aims to label rat bone marrow mesenchymal stem cells with superparamagnetic iron oxide

then observe whether the magnetic field could induce labeled cells targeted migration in vitro. 【Method】 Rat bone marrow mesenchymal stem cells were labeled with superparamagnetic iron oxide and evaluated the labeling efficiency by Prussian blue staining. The viability and proliferation activity of labeled stem cells were evaluated with trypan blue staining and MTT assay respectively. Wound healing assay and transwell culture system were used to test the migration effect of external magnetic field on labeled stem cells.【 Results】 Prussian blue staining showed that mesenchymal stem cells labeled superparamagnetic iron oxide close to 100%. Trypan blue staining and MTT assay were no significant difference between labeled group and control group(P > 0.05). External magnetic field can accelerate the labeled stem cells to fill the blank areas in the wound healing and increase the number of cells crossing the membrane in the transwell culture system(P < 0.05)

while without the magnetic field labeled cells and unlabeled cells were no difference in migration rate(P > 0.05). 【Conclusion】 External magnetic field can induce bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide to migrate with target in vitro.