【Objective】 To investigate the correlation between synthesis diagnostic index (SDI) of diffused optical tomography (DOT) with ultrasonography (US) localization and microvessel density (MVD) based on CD105

as well as their relationship with clinic pathological characteristics of breast cancer. 【Method】 A new machine of diffused optical tomography (DOT) with ultrasonography (US) was used in diagnosis of 273 cases with breast cancer

in which 131 cases were benign and 142 cases were malignant. The machine generates a synthesis diagnostic index (SDI) as a quantitative measure of the likelihood of a lesion being malignant. The receiver operating characteristic (ROC) curve analysis was used to estimate the optimal diagnostic threshold of breast cancer. The microvessel density (MVD) based on CD105 was detected by immunohistochemistry on all the 142 cases of breast cancer. 【Results】 The mean SDI values were 109 ± 48 for benign cases and 191 ± 60 for malignant cases. There was significant difference between them(P < 0.05). According to ROC curve analysis

the areas under ROC curve of SDI was 0.889. The SDI has linear correlation with MVD of breast cancer(P < 0.05). The mean values of SDI and the MVD were significantly correlated with the tumor size

lymph node status

clinical TNM stage and the expression levels of CerbB-2 (P < 0.05)

while they showed no correlation with the expression levels of ER and PR(P > 0.05). 【Conclusion】 SDI of diffused optical tomography with ultrasonography is closely related to microvessel density in breast cancer

which can indirectly reflect the angiogenic activity. It also might play a great role of early diagnosis and in distinguishing benign and malignant tumors.