Abstract:【Objective】This study was designed to establish induced pluripotent stem cell line from Cdyl knockout mice. 【Methods】 Cdyl-/- MEF were attained by adhesion culture of E18.5 embryonic tissue. pMx-Oct3/4

Sox2

c-Myc

Klf4

and hrGFP plasmid were transfected into Plat-E cells

respectively. Then virus-containing supernatants were harvested to infect Cdyl-/-MEF. The characteristics of Cdyl-/- iPSC were analyzed by detection of pluripotent marker expression and differentiation capacity in vivo and in vitro.【Results】 The Cdyl-/- iPS cell colonies were emerged 5 days after Cdyl-/- MEF were infected with virus-containing supernatants. Cdyl-/-iPSC expressed AKP

Oct3/4

SSEA-1 and were able to differentiate into cell types of three germ layers.【Conclusion】 Cdyl-/- iPS cell line was successfully established and provides a valuable model for studying Cdyl function in mouse embryonic development.