【Objective】 To explore expression alterations of CKIs p16 and p21 and cell proliferation marker Ki-67 in young women with cervical intraepithelial neoplasia(CIN)Ⅱ-Ⅲ.【Methods】A total of 109 women were recruited in our study for cervical lesion treatments. They all had high-risk HPV DNA tests by hybrid captureⅡ(HCⅡ)and their final diagnoses of CINⅡ-Ⅲ by histopathologic tests were obtained after cervical biopsy and operations. They were divided into two groups according to their ages: group of < 35 years old and of ≥ 35. Then p16

p21 and Ki-67 expressions were determined by immunohistochemical tests. 【Results】High-risk HPV DNA was detected in all women with positive rate of 100% and high-risk HPV DNA load showed no significant difference between two groups[group of <35 years old:(498±848)pg/mL

group of ≥ 35:(548 ± 756)pg/mL

P > 0.05]. And significant difference didn't exist in p16 expression

either(< 35:100%

≥ 35:96.72%

P > 0.05). While compared with group of ≥35 years old (p21:39.34%

Ki-67:75.41%)

positive rates of p21 and Ki-67 in group of < 35 were increased and differed statistically (p21: 60.42%

Ki-67:91.67%

P < 0.05). 【Conclusion】 Among women with CIN

p21 and Ki-67 expressions in cervical lesions are more frequent in young women. For them

p21 and Ki-67 tests may be added and positive results may predict progressive lesion.