【Objective】 The detection of peripheral leucocytes expression profile between normal pregnancy and preeclampsia at 16-20 gestation weeks

and compare the gene expression difference between them with using DNA microarray technique and human genome cDNA chip. And to seek the factors inducing disorders of lipid metabolism in preeclampsia by testing the difference of lipid metabolism-related genes expression. 【Methods】 A cohort of nulliparous women was recruited. Preeclampsia developed in 6 patients with severe preeclampsia (research group) were diagnosed according to the criteria (Obstetrics and Gynecology

the Sixth Edition)

without complications of internal medicine and other complications of pregnancy)

40 normotensive controls women were matched (control group). To get detection of peripheral leucocytes expression profile at 16-20 gestation weeks

and compare the gene expression difference between two groups using DNA microarray technique and human genome cDNA chip. To detect the plasma levels of oxidized low density lipoprotein (ox-LDL) by ELISA and the deposition of Lectin like oxidized low density lipoprotein receptor-l (LOX-1) and apolipoproteins A5(APOA5) by RT-PCR. 【Results】 The results of microarray analysis of mRNA in peripheral leucocytes between two groups: there were 983 differentially expressed genes in gene chip

in which 719 up-regulating genes and 264 down-regulating genes. The plasma levels of ox-LDL and LOX-1 were significant higher in study group than that in control group (P < 0.05)

the level of APOA5 were significant lower in study group than that in control group (P < 0.05)

the level of LOX-1 was positively correlated with the plasma concentration ox-LDL(R = 0.91

P < 0.05). 【Conclusion】 oxLDL

LOX-1

and APOA5 gene expression of peripheral leucocytes in pre-eclampsia patients is significantly different from that of normal pregnancy.