【Objective】 The nephrotoxic serum nephritis animal model was constructed to investigate the therapy effect of glial cell linederived neurotrophic factor (GDNF) overexpression of bone marrow mesenchymal stem cell and improving renal function to the nephrotoxic serum nephritis(NSN)

and the clinical efficacy was evaluated. 【Methods】 MSC was transfected with adenovirus expression vector containing GDNF

evaluate the transfection efficiency. Then MSC with overexpressing GDNF was injected to rats through the left renal artery

pathological and biochemical changes were detected to see the improvement of renal function. MSC with GDNF overexpression were located and counted in kidney tissue by fluorescence experiments to evaluate its efficacy. 【Results】 After infusion

the MSC were located in the renal cortex

serum creatinine

uremic nitrogen and urinary protein of NSN rats infused pAdGDNFGFP statistically decreased compared with control group

renal function was statistically ameliorated

P < 0.05;pathology results showed that the GDNF overexpressing group had lower renal damage score than the control group

P < 0.05. 【Conclusion】GDNF well improved renal function and pathological changes in NSN rats

the protection of GDNF overexpressing mesenchymal stem cells to NSN rat kidney provided a kind of new idea to for various diseases such as NSN.