【Objective】 To study the epidemiology of respiratory syncytial virus (RSV)

human rhinovirus (HRV)

influenza virus type A (flu A)

influenza viruses type B (flu B)

human metapneumovirus (Hmpv)

adenovirus (ADV)

parainfluenza virus (PIV) and human coronaviridae (HCoV) in patient with acute respiratory infection in Guangzhou. 【Methods】 By multiplex RT-PCR

the above 8 virus were detected in the nasopharyngeal swabs of patient with acute respiratory infection who came to the clinic of the number two hospital of Guangzhou medical college during September

2006 to August

2009. And analysis was made to the distribution of season

year and age. 【Results】 1388 nasopharyngeal swabs were collected in the 3 years

and the positive rate of virus detection was (40.634%)

with RSV the most common virus detected (17.8%). The rate of RSV detected decreased each year (P = 0.000)

while HMPV increased in the second year (P = 0.016). There was some difference on the distributing of time of the virus. The infection rate of RSV was high in 2007

and it increased in spring over the 3 years (R = 25.2%

P = 0.001). The infection rate of HRV increased in autumn (P = 0.001). The infection rate of PIV increased in spring (P = 0.000)

and decreased in summer (P = 0.023) and winter (P = 0.048). The infection rate of flu A increased in summer (P = 0.000). The infection rate of HMPV increased in spring (P = 0.05) and autumn (P = 0.01). And there was also some difference on the infection rate between different viruses in the same season. The infection rate of RSV (P = 0.000) and HRV (P = 0.004) were higher than other virus in spring. In summer

the infection rate of HRV (P = 0.000) and flu A(P = 0.000) were higher. In autumn

the infection rate of HRV was higher (P = 0.000). And in winter

the infection rate of HRV (P = 0.002) and RSV(P = 0.000) were higher. The infection rates of virus differ from different population. The infection rate of RSV (P = 0.004)

HRV (P = 0.029) and HMPV (P = 0.001) increased in children. The infection rate of ADV (P = 0.000) and Flu B (P = 0.011) increased in population of age of 3 ~ < 14. The infection rate of Flu A increased in population of age of 14 ~ < 40 (P = 0.011). 【Conclusion】 RSV was popular in spring

Flu A was popular in summer

and the infection rate of HRV was high through the whole year. RSV

HRV

and HMPV was popular in infant

ADV and Flu B were popular in population of age of 3 ~ < 14

and Flu A was popular in population of age of 14 ~ < 40. RSV was one of the most common virus detected

with its infection rate of high in 2007.