【Objective】To survey the prevalence of human papillomavirus (HPV) and the type distribution in the women with and without cervical lesion from Guangzhou city. 【Methods】The women who anticipated cervical cancer screening with normal cytology were grouped as normal LCT group

histologically confirmed cervical intraepithelial neoplasm grade I (CIN 1) as low-grade intraepithelial lesion (LSIL)

CIN2-3 as high-grade intraepithelial lesion (HSIL) and invasive cervical cancer as ICC. Samples were detected by flow-through rapid hybridization. The set had a capacity of detecting 21 genotypes including high-risk HPVs (13 subtypes including HPV-16

18

31

33

35

39

45

51

52

56

58

59 and 68)

low-risk HPVs (5 subtypes including HPV-6

11

42

43

and 44)

and other types (3 subtypes including HPV-53

66

and cp8304). 【Results】A total of 971

86

286

and 97 women with normal cytology

with CIN 1

with CIN 2-3

and with ICC were enrolled

respectively. Overall HPV prevalences were 9.6%

62.1%

82.3%

and 90.2%

respectively

in women with normal cytology

with LSIL

with HSIL

and with ICC. High-risk HPV prevalences were 7.2%

50.2%

80.6%

and 87.4%; Low-risk HPV prevalences were 2.1%

6.0%

9.2%

and 5.4%; other HPV prevalences were 1.4%

4.7%

8.0%

and 8.5%. Stratified by cervical lesion grade

the five most common prevalent HPVs and type-specific prevalence were HPV-52(2.7%)

16(1.3%); 31(1.2%)

58(1.1%)

cp8304 (0.9%) in the women with normal cytology; 52(23.3%)

16(11.6%)

68(9.3%)

33(7.0%)

and 53(7.0%) in the women with LSIL;16(39.9%)

58(7.7%)

33(6.3%)

18(5.6%)

and 52(4.9%) with HSIL; 16(52.6%)

18(21.6%)

58(9.3%)

52(6.2%)

cp8304(4.1%) with ICC. 【Conclusion】Overall HPV prevalence in the women from Guangzhou with normal cytology

LSIL

HSIL

and ICC were similar to that reported in China and increased with severity of cervical lesion. But HPV-52 was the most predominant prevalence in the women with normal cytology and LSIL instead of HPV-16/18. HPV-16/18 turned to be predominant prevalence in the women with HSIL and ICC and may played important roll in progressing to ICC.