【Objective】To explore the homing characteristics of bone marrow mesenchymal stem cells (MSCs) in the allogeneic splenocytes sensitized mice.【Method】After normal allogene bone marrow MSCs were cultured and evaluated

MSCs were labeled by 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) in vitro. After injected CFSE labeled MSCs (CFSE+ cells) through vena caudalis in 6h

24h

and 48h

homing trace were explored in the different organs both in the sensitized and normal mice group (each group 10 samples mice) through frozen section and flow cytometry (FCM) detection methods.【Results】Both in the methods of frozen section and FCM

we discovered in the sensitized mice group MSCs were aggregated and homing in spleen and liver quickly and lastingly

while in the normal mice group MSCs were homing to the bone marrow in the end. Among those FCM quantitative result

in 6h the percentage of spleen CFSE+ cells in the sensitized mice group was (3.78±0.66)%

significant difference was found in spleen between the sensitized and normal group at the same time (P <0.05); while in 48 h

the percentage of bone marrow CFSE+ cells in the sensitized and normal mice group were (0.43±0.35)% and (5.40±1.58)%

respectively (P <0.05). 【Conclusion】 Immunity organs such as liver and spleen

influenced by the sensitized of allogeneic splenocyted transfusion

had the orientating function in the normal allogene bone marrow MSCs homing

which Interrupted the homing to the bone marrow in the normal condition.