【Objective】To investig ate the relationship betw een serum markers of liver f ibrosis and liver histopathologic changes in patient s w ith chronic hepatitis .【Methods】The measurement of serum levels of hyaluronic acid (HA)、serum ty pe Ⅲ procollagen (PC Ⅲ)and collagen Ⅳ(Ⅳ-C)w as carried out by radioimmunoassay in 191 patients w ith different chronic hepatic diseases .These levels w ere compared w ith the histopathological g rading and staging of those patients .【Result s】The serum level of HA w as signif icantly hig her in G4 and S4 than that in o ther g rading g roups and staging groups .The histopathological g rading and staging w ere closely correlated with the serum level of HA .The serum level of PC Ⅲ w asn' t signif icantly increased in acco rdance w ith the histopathological grading and staging .The serum level of Ⅳ-C was signif icantly higher in G4 than that in other grading g roups and was closely correlated wi th the histopathological grading but not with the staging .【Conclusions】HA serum levels is found to be a useful marker of the liver fibrosis .PC Ⅲ and Ⅳ-C are not related to the staging of the liver fibrosis .Thus PC Ⅲ and Ⅳ-C can not be used as substitutes for liver biopsy in diagnosis of liver fibrosis .