【Objective】To investigate the effects of Epstein-Barr virus（EBV）on proliferation，apoptosis and lipid metabolism of gastric cancer cells ，revealing the pathogenesis and development of EBV- associated gastric carcinoma（EBVaGC）. 【Methods】 EBV-positive cell line AGS-EBV was established by co-culturing AGS and Akata. Then we compared proliferation ，apoptosis and lipid metabolism level between AGS and AGS-EBV cells. CCK- 8 assays and Annexin V PE/7-AAD assays were performed to determine the proliferation and apoptosis of gastric cancer cells. Oil-Red O staining and three kinds of kits were used to detect lipid contents including lipid droplets，free fatty acid，triacylglycerol and total cholesterol. Key enzymes of lipogenesis were measured by qRT-PCR.【Results】EBV promoted the proliferation of gastric cancer cell line AGS（F=23.214，P=0.001；P values of 24 h，48 h，72 h，96 h and 120 h were 0.007，0.004，＜0.001，＜0.001 and ＜0.001，respectively），inhibited apoptosis（P values of late and total apoptotic rates were 0.032，< 0.001），and increased intracellular lipid droplets，free fatty acids（P<0.001），triacylglycerol（P=0.004） and total cholesterol（P<0.001）probably via lipogenesis.【Conclusions】EBV promotes proliferation，inhibits apoptosis， and enhances lipid metabolism of gastric cancer cells.