最新刊期
      卷 36 ， 期 2 ， 2015
      • Vol. 36, Issue 2, (2015)
        关键词：Ebola virus (EBOV);Ebola hemorrhagic fever   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To evaluate the potential association of the molecular characteristics of avian influenza A H7N9 virus with its pathogenicity and patients’ clinical outcome by comparing the molecular differences between the two avian influenza A H7N9 virus strains isolated from the fatal case and the patient showing mild disease with full genome sequences, so as to provide further evidence for better understanding of the pathogenesis of avian influenza A H7N9 virus. 【Methods】 The two virus strains isolated from the fatal case and the patient showing mild disease were subjected to whole genome sequencing. The genome characteristics, the evolutionary relationship, the receptor binding sites, host specificity, virulence, drug resistance and potential glycosylated sites were analyzed by bioinformatics software. The sequences of avian influenza A H7N9 viruses isolated from the deceased and the recovered patients during 2013 and 2014 were downloaded from The Global Initiative on Sharing All Influenza Data (GISAID), and the pivotal sites were compared to elucidate their relationship with the pathogenicity and the clinical outcome. 【Results】 The HA?NA and M gene of the two virus strains clustered with original novel avian influenza A H7N9 virus circulating in East China in 2013, while the NP, NS, PA, PB1 and PB2 gene originated from avian influenza H9N2 virus circulating in the poultry of Guangdong in 2013 . The mutation rate of L336M in PA and I368V in PB1 were increasing with time and had geographic differences. The two virus strains had over 99.2% nucleotide identity and 99.5% amino acid identity, but also showed several amino acid differences. Receptor binding sites exhibited one amino acid difference(226L and 226Q). Site 591 and site 627 in PB2 related to host specificity were different (591L and 591Q, 627E and 627K). Site 353 in PA and site 627 in PB2 associated with virulence were distinct (353R and 353K, 627E and 627K). The M2 protein of both virus strains had S31N mutation. All potential glycosylation motifs were relatively conservative. The mutation rate of PB2-E627K substitution of all the avian influenza A H7N9 viruses isolated from the fatal cases reported from 2013 until October 2014 was higher than the viruses isolated from the recovered cases. 【Conclusions】 The emergence of the two avian influenza A H7N9 virus strains in Guangdong may due to the further reassortments between the original novel avian influenza A H7N9 virus circulating in East China and the avian influenza H9N2 virus circulating in the poultry of Guangdong. The avian influenza A H7N9 virus was evolving because of the frequent reassortments and the increasing and geographic mutations of several key sites. Therefore, it is necessary to monitor its evolution and prevent a potential outbreak. The two virus strains, with different clinical outcomes, shared high sequence similarity, but also had differences in some pivotal sites. The mutation E627K in PB2 might be associated with the pathogenicity and the clinical outcome.  
        关键词：avian influenza A H7N9 virus;genome sequencing;molecular characteristics;molecular evolution;pathogenicity;clinical outcome   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        关键词：Ginsenoside Rb1;natural aging;mouse;brain tissue;mTOR/p70S6K   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To observe the effects of in vitro exogenous administration of ghrelin on LPS induced apoptosis in rat alveolar macrophage NR8383, and to investigate the potential role of JNK signaling pathway in this impact. 【Methods】 CCK-8 assay was used to examine the cell viability of NR8383 treated by LPS alone or co-incubated by ghrelin and LPS; flow cytometry and TUNEL were used to assessed the apoptosis rate; expression of JNK, phospho-JNK, Bax, Bcl-2, cleaved caspase-3 were detected using Western blot analysis. Laser scanning confocal microscopy was used to observe the phagocytosis of NR8383. Ghrelin receptor antagonist [D-Lys-3]-GHRP-6 and JNK specific inhibitor SP600125 were used to inhibit ghrelin receptor and JNK activation respectively. 【Results】 LPS significantly inhibited the proliferation of NR8383 in a dose-dependent manner, with IC50 value of 250 μg/mL. However, ghrelin can block this inhibition casused by LPS. Flow cytometry and TUNEL analysis showed that ghrelin could decrease the apoptosis induced by LPS in NR8383 (P < 0.05), while application of ghrelin receptor antagonist [D-Lys-3]-GHRP-6 weakened the antiapoptosis effect of ghrelin(P < 0.05). LPS activated JNK kinase, increased the expression of Bax and cleaved caspase-3, but decreased Bcl-2 level, which was significantly reversed by pretreatment with ghrelin(P < 0.05). Ghrelin also apparently maintained the phagocytosis of NR8383 post LPS treatment (P < 0.05). 【Conclusion】 Ghrelin inhibited LPS induced NR8383 apoptosis by down-regulating JNK signal pathway, and maintained the phagocytosis of NR8383.  
        关键词：ghrelin;apoptosis;LPS (lipopolysaccharide);alveolar macrophage;JNK(c-Jun N-terminal kinase)   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate the effect of angiotensin II (AngII) on the expression and the translocation of Glucose Transporter-1 (GLUT-1) in cultured mouse podocyte cells. 【Methods】 Method one, The expression of mRNA and protein of GLUT1: Cultured mouse podocyte cells in vitro were randomly divided into normal control group(C), AngII at the concentration of 10-10 mol/L (low concentration group),10-8 mol/L (middle concentration group) ,10-6 mol/L (high concentration group). The expression of mRNA and protein of GLUT1 were detected by semi-quantitative RT-PCR and Western blot respectively. Method two, the distribution of GLUT1 in the plasma membrane (translocation) of podocyte: Podocyte Cells were divided into normal control group (C), insulin 10-6 mol/L, AngII 10-6 mol /L plus insulin 10-6 mol/L and losartan (LO) 10-6 mol/L plus AngII 10-6 mol/L and insulin 10-6 mol/L groups. The way of Western blot and immunocytofluorescence were used. 【Results】 Compared to control group, AngII at the concentration of low, middle, and high groups increased GLUT1 transcription by 5.89% (P 0.05), and by 11.33% in the LO 10-6 mol/L plus AngII 10-6 mol /L and insulin 10-6 mol/L group (P < 0.01). Immunocytofluorescence indicated the expression of GLUT1 in the plasma membrane of insulin group was the strongest in the four groups and losartan could increase the GLUT1 expression in the plasma membrane partly. 【Conclusion】 AngII increases the expression of GLUT1, which is concentration dependent. Ang II prohibits the insulin-induced increasing of GLUT1 in the plasma membrane (translocation), and losartan could partly blocks the effect of AngII on the function of insulin.  
        关键词：podocyte;glucose transporter 1;angiotensin II;losartan   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        关键词：colorectal cancer;KRAS;RASGRF1   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To try to find an optimal time to vitrify biopsied blastocyst using mouse model. 【Methods】 258 mouse blastocysts were divided into four treatment groups and two control groups (biopsy control group (n = 48) and vitrification control group (n = 29). Biopsied blastocysts were cryopreserved after different time of in vitro culture [0 h (n = 43), 0.5 h (n = 39), 1 h (n = 50), 2 h (n = 49)]. Blastocyst cryo-survival rate, blastocele re-expansion rate and blastocyst hatching rate were compared among groups. The mean total blastocyst cell number, apoptotic cell number, DNA integrity index and the apoptotic cells at biopsied site were counted. 【Results】 The cryo-survival rate, blastocele re-expansion rate and hatching rate were not statistically different among groups; The mean total blastocyst cell number, apoptotic cell number, DNA integrity index and the apoptotic cells at biopsied site were not statistically different among groups too. 【Conclusion】 In vitro culture before vitrification did not show any beneficial effect for the cryopreservation of biopsied blastocysts. Cryopreservation of biopsied blastocysts could be carried out flexibly in IVF lab after biopsy manipulation.  
        关键词：blastocyst;biopsy;vitrification;cryo-injury;apoptosis   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】To report a Chinese family with distal hereditary motor neuropathy(dHMN), to conduct molecular genetics study to identify the pathogenic gene and to explore the applications of exome sequencing in clinical diagnosis and research. 【Methods】 Clinical and electrophysiological examination was performed, the proband and a collateral patient were subjected to exome sequencing, bioinformatics analysis, then the 22 family members whose blood sample were collected include 10 patients were subjected to co-segregation analysis by mutation screening. 【Results】 The results of exome sequencing and PCR sequencing showed that all patients have the mutation of glycosylation site in the 3rd exon of BSCL2 gene: p.Ser90Leu, which was first reported domesticly. 【Conclusion】 Our study confirms that exome sequencing is a comprehensive and feasible approach to diagnose dHMN. And for other high genetic heterogeneity diseases, exome sequencing is simpler and more convenient than normal PCR-direct sequencing.  
        关键词：exome sequencing;dHMN-V;BSCL2 gene   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objectives】 To study the clinical significance and underlined mechanisms of vasculogenic mimicry (VM) in adrenocortical carcinoma (ACC).【Methods】 Thirty-nine formalin-fixed and paraffin-embedded samples of ACC diagnosed between January 1999 and March 2014 were collected and stained for CD34, periodic acid and Schiff (PAS), and vascular endothelial growth factor receptor-2(VEGFR-2). Clinicopathologic data of the patients was analyzed. Exact probability method was used to analyze the relationship between VM,VEGFR-2 and clinicopathologic parameters of ACC.【Results】 VM was detected in 17 of the 39 ACC samples. The VM in ACC was associated with Weiss score(P = 0.017), TNM stage(P = 0.024), and VEGFR-2(P = 0.012), but not with the patient’s gender, age, tumor location, hormone secretion, or tumor size(cm).The median overall survival time of patients with VM-positive and VEGFR-2-positive was significantly shorter than that of the patients with VM-negative andVEGFR-2-negative tumors(P < 0.05). Single factor analysis showed that age, tumor size, Weiss score, TNM staging, VEGFR-2-positive, VM-positive may affect the prognosis of ACC patients. Furthermore, the multivariate analysis demonstrated that VM were independent risk factors for the prognosis of patients with ACC. 【Conclusions】 VM was presented in ACC tissues, and was associated withVEGFR-2 overexpression. This may indicate that the relative molecular pathways could be potential therapeutic targets for ACC.  
        关键词：adrenocortical carcinoma;vasculogenic mimicry;vascular endothelial growth factor receptor-2   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate the expression of negative costimulatory molecule PD-L1 in non-muscle invasive bladder cancer (NMIBC) and its effect on intravesical instillation therapy after surgery. 【Methods】 10 normal bladder tissues and 131 bladder cancer tissues were available from patients underwent TUR-Bt at our institution between January 2006 and March 2013. NMIBC patients were administrated with epirubicin or Picibanil for adjuvant intravesical instillation therapy. Immunohistochemistry staining was used to detect the PD-L1 expression in tumor and normal specimens. All data of the time and number of tumor recurrence and progression were recorded. 【Results】 PD-L1 expression were negative in normal bladder 0(0/10),comparing with 56.5%(74/131)in NMIBC (P = 0.000) . The expression of PD-L1 in pTa and pT1 bladder cancer were 35.14%(13/37)and 60.64%(57/94),respectively (P = 0.008). The tumor recurrence and progression rates in PD-L1(+) patients were 37.8% and 10.8%,which were higher than 12.3% and 3.5% in PD-L1(-) patients(P 0.05). 【Conclusions】 Expression of negative costimulatory molecule PD-L1 in NMIBC is significantly correlated with pathologic stage and prognosis. Detecting the PD-L1 might help determine the optimal instillation agents for NMIBC patients.  
        关键词：non-muscle invasive bladder cancer;PD-L1;intravesical instillation therapy;tumor immune escape   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate the effectiveness of luteinizing hormone (LH) in decreasing serum vascular endothelial growth factor (VEGF) level during controlled ovarian hyperstimulation (COH). 【Method】 Comparing serum VEGF level on COH starting day and comparing elevated serum VEGF level on human chorionic gonadotropin (HCG) administration day, oocyte pick up (OPU) day and embryo transfer (ET) day between 20 patients with whole course 225 U LH application and 50 patients without during COH. 【Results】 Serum VEGF level on starting day and serum VEGF level elevated on HCG day was comparable of two groups. However, serum VEGF level elevated on OPU day (P = 0.017) and ET day (P = 0.009) of study group were significantly lower than control group. 【Conclusion】 Whole course 225 U LH application during COH can effectively decrease serum VEGF level on OPU and ET day.  
        关键词：controlled ovarian hyperstimulation;luteinizing hormone;vascular endothelial growth factor   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To compare the efficacy and safety of nasal intermittent positive pressure ventilation (NIPPV) with nasal continuous positive airway pressure (NCPAP) in early very low birth weight preterm infants. 【Methods】 A prospective randomized study was performed. The gestational age 26+0 ~ 32+0, birth weight≤ 1 500 g infants were randomized to NIPPV or NCPAP group soon after birth. The clinical outcomes were compared. 【Result】 200 patients were involved in the study. The rates of BPD did not differ significantly between the NIPPV and NCPAP groups (39% vs 45%, P > 0.05) Infants who received NIPPV treatment within the early 72 hours of life had less frequently required intubation(23% vs 41%, P < 0.05) and less pulmonary surfactant treatment. (33% vs 48%, P < 0.05). The duration of endotracheal mechanical ventilation and duration of supplemental oxygen were all less in NIPPV group. Other adverse neonatal outcomes did not differ significantly between two groups. 【Conclusion】 Early NIPPV did not significantly reduce BPD, as compared with NCPAP. However, it is another effective and safe respiratory supportifor VLBW preterm infants.  
        关键词：very low birth weight;preterm infant;nasal intermittent positive pressure ventilation;nasal continuous airway pressure;bronchopulmonary dysplasia   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To explore the influence of UPOINT domains on erectile dysfunction (ED) in patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). 【Method】 From January to July 2014, 192 CP/CPPS patients were evaluated by UPOINT system. Each patient underwent clinical assessment and answered the questionnaire consisted of socio-demographics, Patient Health Questionnaire (PHQ), Pain Catastrophizing Scale (PCS), National Institutes of Health- Chronic Prostatitis Symptom Index (NIH-CPSI) and International Index of Erectile Function (IIEF-5).【Results】 Of 192 CP/CPPS patients, 47.4% suffered from ED. The positive rate was U 70.8%, P 59.9%, O 80.7%, I 23.4%, N 45.3% and T 62.5% respectively for each UPOINT domains. Multivariate logistic regression showed UPOINT psychological (P) domain was independent risk factor for ED in CP/CPPS (OR=3.749,95% CI 1.903-7.384,P 0.05). 【Conclusion】The CP/CPPS patients had a higher rate of ED. Psychological factor was independent risk factors of ED in CP/CPPS. Pain, voiding symptoms and local inflammation of the prostate were not. But severe pain and voiding symptoms affected patients’ psychological state, which affected the patient’s erectile function.  
        关键词：chronic prostatitis/chronic pelvic pain syndrome;erectile dysfunction;UPOINT system;risk factors   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate serum levels of CCL-17, CCL-27, TNF-α and IL-10 in the patients with nonimmediate drug hypersensitivity reactions (niDHR) and evaluate the correlation among the cytokines, exploring their functions in pathogenesis of niDHR. 【Methods】 Sixty patients with niDHR, including 30 patients with maculopapular exanthema (MPE) and 30 patients with Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN), and 60 healthy donors were recruited into the study. Serum CCL-17, CCL-27, TNF-α, and IL-10 levels were determined by enzyme-linked immunosorbent assay. 【Results】 Serum levels of CCL17, CCL27, TNF-α, and IL-10 were all significantly elevated in patients with niDHR compared to normal controls (P < 0.001; P < 0.001; P < 0.001;P < 0.001). The levels of CCL17, CCL27, TNF-α, and IL-10 were found to be higher (P < 0.001; P < 0.001; P < 0.001; P < 0.001) in the patients with MPE than normal controls. Serum levels of CCL27, TNF-α, and IL-10 were significantly elevated in the patients with SJS/TEN (P < 0.05; P <0.001; P < 0.001) compared with control group. In all sixty patients with niDHR, serum CCL17 levels were positively correlated with CCL27 levels (r = 0.371, P < 0.05), but serum TNF-α levels were negatively correlated with CCL17 levels (r = -0.513, P < 0.05). 【Conclusion】 The result indicates CCL-17, CCL-27, and IL-10 participating in the pathogenesis of niDHR. The chemokines have different ability to attract different T-cell subtypes. TNF-α might play a mediated role among chemokines in niDHR.  
        关键词：nonimmediate drug hypersensitivity reactions;CCL-17;CCL-27;TNF-α;IL-10   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 Based on Theory of Planned Behavior to investigate the characteristics of the cognitive behavior on female heroin abusers for the clues of treating their abuse behaviors. 【Methods】 Adult Nowicki-Strickland Internal-External Control Scale, General Self-Efficacy Scale and Single Category Implicit Association Test were used in randomized controlled study to select 277 female heroin abusers and 117 matched females. 【Result】 Normative beliefs of subjects from the research and the control groupshave significant difference(P < 0.05); The Self-Control score of research group was higher than control group(P < 0.001); Thescores of IAT between the research and the control groups have significant difference(P < 0.001). 【Conclusion】 The female heroin abusers are in a social environment ofrelatively tolerant to the action of drug taking compared with normal female, whose attitude of drug taking tend to be neutral and self-control tend to be external.  
        关键词：locus of control;implicit attitude;female;heroin addicts;control of belief;Theory of Planned Behavior   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To compare the two kinds of strategy of preimplantation genetic diagnosis for carriers with chromosomal translocation, in order to have a feasible strategy. 【Methods】 A total of 183 PGD cycles for chromosomal translocation were performed from April 2012 to June 2014, including 91 FISH-PGD cycles using blastomere biopsy and fresh embryo transfer,called FISH group, and 92 SNP-PGD cycles using blastocyst biopsy and thawed embryo transfer, called SNP group. To compare the diagnosis and clinical outcome with the two kinds of strategy. 【Results】 In PGD results, the biopsy cycles of no embryo transfer, SNP group were lower than FISH group, there was statistical significance. The normal rate in SNP group was 33.8%, higher than FISH group,there was statistical significance in normal rate. The clinical pregnancy rate per embryo transfer in SNP group was higher than FISH group, yet the misscarrage rate or embryo damage rate was lower than them. 【Conclusion】 The PGD strategy of applying blastocyst biopsy, SNP, embryo cryopreservation and thawed ET helps to have a better clinical outcome. It may be a promise choice of future PGD treatment for carriers with chromosomal translocation.  
        关键词：chromosomal translocation;preimplantation genetic diagnosis;fluorescence in situ hybridization;single-nucleotide polymorphism array   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 This study aimed to investigate the value of MRI to evaluate knee stability after anterior cruciate ligament reconstruction.【Methods】 Clinical and MRI study were performed on 158 eligible patients with anterior cruciate ligament reconstruction, bone tunnel, graft and internal fixation device MR imaging were analyzed and compared with knee stability results by clinical examination. According to results of clinical examination, 26 cases were diagnosed instability (positive group) and 132 cases were stability (negative group). The changes of the tibial and femoral tunnel diameter, differences of positive rate in bone tunnel position, graft and internal fixation between two groups were compared. Kappa test for consistency between clinical examination and MR performance in bone tunnel position, graft and internal fixation. Sensitivity, specificity, accuracy, positive and negative predictive values were calculated for MRI diagnosing knee joint instability. 【Results】 Inner diameter changes of the tibial and femoral tunnel in group instability were greater than group stability, the difference was statistically significant (P < 0.01). There was a higher positive rate in positive group than negative group with MR sign of bone tunnel position, graft performance and internal fixation (P < 0.001), Kappa value was 0.599, 0.744, 0.285, respectively, P < 0.001, statistical differences between the two groups.【Conclusions】 There was certain value of MRI in assessing knee stability after anterior cruciate ligament reconstruction, which may provide valuable information for clinical post-operation follow up and second operation.  
          
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To study the optical intrinsic signal(OIS) imaging of peri-infarct depolarizations (PID) in mice and to investigate the influence of knockout of Kir6.1 on PID.【Methods】Kir6.1 knockout mice and wild type littermates were subjected to MCAO by standard intraluminal filament method. The main characteristics of PID were studied by 4-wavelength OIS imaging technique. 【Results】 PID were identified as consistent, red and blue interaction waves in cortical reflectance that slowly propagated peripherally from the origin site. There were 4 patterns of PID propagation including rostro-caudal, latero-medial, caudo-rostral, and contralateral pattern. Kir6.1 knockout mice had a mean of 25.5 ± 6.5 PID which was much higher than a mean of 15.5 ± 4.8 in wild type mice (P 0.05). Kir6.1 knockout mice showed an increased mean infarction volume (38.2 ± 7.5%) compared with wild type mice (27.8 ± 6.6%). 【Conclusion】 Using 4-wavelength OIS system, we are able to get high temporal-spatial resolution color images which are useful for analyzing temporal-spatial characteristics of PID in detail. Knockout of Kir6.1 facilitates induction of PID in focal cerebral ischemia, perhaps owing to decreasing spatial potassium buffering ability of astrocytes in kir6.1 knockout mice. Furthermore, more PID may contribute to the increased infarction volume in kir6.1 knockout mice.  
        关键词：brain infarction;spreading depolarization;optical intrinsic signal;kir6.1   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To discuss the diagnostic value of hepatocyte-specific contrast agent Gd-EOB-DTPA in the magnetic resonance imaging examination of hepatic focal nodular hyperplasia (FNH). 【Methods】 In this retrospective trial, 36 patients(including 46 lesions) diagnosed with FNH underwent Gd-EOB-DTPA enhanced MRI examinations. Analyze their imaging findings on nonenhanced, multiphase enhanced imaging and Diffusion Weighted Imaging (DWI). 【Results】 ①all of the 46 lesions show obvious enhancement in arterial phase, whose signal are much higher than the background liver parenchyma, 6.52% of which can see tortuous vessels in or around the lesion, and they all become isointense or slightly hyperintense in portal venous phase and late phase except the central scar. ②the signal of lesions in hepatocellular phase has 5 types: homogeneously hyperintense, inhomogeneously hyperintense, isointense, peripheral hyperintense with a hypointense center and hypointense. ③in the 46 lesions, 16 has central scar, and the diameter of 10 (62.50%, 10/16) is larger than 30 millimeter. 13 of the 16 central scars show typical signal characteristics in T1 weighted imaging and T2 weighted imaging, 13 show enhancement in portal venous phase, and all become hypointense in hepatocellular phase. ④Between the 36 patients, 12 patients (20 lesions) have DWI, 5 of the which show isointense,14 show slightly hyperintense and 1 show hypointense. The mean ADC value of all the lesions and the background liver parenchyma is (1.17 ± 0.047) × 10-3 mm2/s and (1.24 ± 0.039) × 10-3 mm2/s (P = 0.199 > 0.05) separately, so the difference between them has no statistical significance. 【Conclusion】 Most FNH are isointense or hyperintense in hepatocellular phase, which contributes to the diagnosis. While the difference of ADC value between FNH and the background liver parenchyma has no statistical significance.  
          
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 The aim of this study was to explore the relationship between CA19-9 and clinicopathological characteristics in endometrial cancer. 【Methods】 2342 patients source from cooperative?hospitals for endometrial cancer prevention and control project with surgically treated endometrial carcinoma preoperatively evaluated with serum levels of CA19-9 were included in this retrospective study. 【Results】 The serum CA19-9 positive rates in endometrial cancer increased with stages which were 17.4%, 32.9%, 39.5% and 44.3% for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ, respectively (P 0.05), but related with tumor grade, deep myometrial invasion, abdominal dropsy cytology, cervical stroma involvement, lymph-vascular space invasion, adnexal metastasis and lymph node metastasis (P < 0.05). Multivariate analysis revealed that poorly differentiated, deep myometrial infiltration, cervical involvement and advanced cancer related with positive serum CA19-9 (P < 0.05). 【Conclusions】 preoperative serum CA19-9 level was a valuable index in prediction pathological high risk factors of endometrial cancer.  
        关键词：endometrial cancer;CA19-9;risk factors   
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        发布时间：2023-12-12
      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To assess the value of plasma miR-499 to predict sepsis with myocardial damage. 【Methods】 The blood specimens from 112 patients with sepsis were collected after admission. According to the occurrence of myocardial damage, they were classified into sepsis with myocardial damage patients (45 cases) and sepsis without myocardial damage patients (67cases). A total of 20 healthy subjects were enrolled as control group. Plasma miR-499 was assessed using real-time polymerase chain reaction. 【Results】 In plasma from sepsis with myocardial damage patients, cardiac myocyte-associated miR-499 was highly elevated, as compared with sepsis without myocardial damage patients and control subjects (P < 0.01). Receiver operating characteristic curve analysis revealed an area under the curve of 0.889 for miR-499. 【Conclusion】 Cardiac damage initiates the detectable release of cardiomyocyte-specific miR-499 into the circulation. Plasma miR-499 can be markers for the diagnosis of myocardial damage after sepsis.  
          
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To add superior items into the “Social Relationships” domain of WHOQOL-BREF by item response theory (IRT). 【Methods】 Nominal group worked interactively with focus group to build the item pool. Superior items were selected using IRT, traditional methods and experts’ importance rating to add into the “Social Relationships” domain. We administered the scale to 700 parents by quota sampling, and estimate the discrimination α, difficulty b1-b4, Item Information Function and Differential Item Functioning (DIF) of items. We also analyzed the property of items according to the dispersion degree, correlation coefficient, factor analysis, Cronbach’s α coefficient,multiple linear regression and experts’ importance rating. 【Results】 Three items D36, F14.3, A1 were selected to be added into “Social Relationships” domain according to the above methods. 【Conclusion】 The revised scale WHOQOL-BREF-R was developed on the strict procedures according to IRT.  
        关键词：IRT;quality of life;scale;item selection   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To provide scientific basis for unifying standard of the reference value of Chinese adults’ pulmonary arterial systolic pressure. 【Methods】 This paper collected the reference value of PASP of 3405 healthy adults from 61 units in China. The relationship between PASP reference value and the 7 geographic factors was studied by correlation analysis and two models were built by regression analysis and factor analysis. Then the optimal model was selected by t test. 【Results】 There was significant correlation between PASP reference value and geographical factors. The factor analysis model was selected as the best one. Then the paper calculated the PASP reference value according to the model, and interpolated the distribution map of PASP reference value by Kriging method in ArcGIS 10.0 software. 【Conclusion】 Altitude is the main impact factor of adults’ PASP reference value. If the geographic factors of a certain area are obtained, the reference value of Chinese adults’ PASP can be calculated by the factor analysis function. In addition, the PASP reference value of an area can be read from the distribution map we got.  
        关键词：pulmonary artery systolic pressure;geographic factors;regression analysis;factor analysis;Kriging   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate the prevalence of metabolic syndrome in obese children and adolescents and the clinical characteristic in outpatient. 【Method】 According to the newest diagnosis criteria of metabolic syndrome in children in china, the databases of the clinical and experimental characteristics in simple obesity children and adolescents diagnosed from 2008 to 2011 in the First Affiliated Hospital of SUN Yat-sen University were analyzed. 【Results】 119 patients with simple obesity were enrolled in the study, all were above 6 years old. 51(42.86%) had at least one component of metabolic syndrome, 47(39.50%) had two components. 21(17.65%) had more than two components of metabolic syndrome. Triglyceride (TG), non-high-density lipoprotein cholesterol (nonHDLC) and homeostasis model assessment-insulin resistance index (HOMA-IR) in three and over components of metabolic syndrome group were significant higher than these of the other groups (P < 0.05). The three groups all were central obesity. Correlation analysis suggests that the level of nonHDLC was positively related with total cholesterol (TC), low density lipoprotein cholesterin (LDC-C), TG and the number of metabolic syndrome component. Non-alcoholic fatty liver disease (NAFLD) was positively related with FINS and HOMA-IR. 【Conclusions】Obese children in outpatient have high prevalence of the metabolic syndrome. Central obesity (CO) may be the initiating factor of metabolic abnormality. NonHDLC was the main dyslipidemia. Insulin resistance, hypreinsulinemia were the main factors causing NAFLD.  
        关键词：obesity;metabolic syndrome;insulin resistance;child;nonHDLC;NAFLD   
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      • Vol. 36, Issue 2, (2015)
        摘要：【Objective】 To investigate gout-related diet control knowledge of patients and identify the target of health education for patients with gout. 【Methods】 175 continuous patients with primary gout were interviewed with self-designed questionnaire including patient?蒺s demographic data, the assessment of disease burden and 12 questions about diet control knowledge. 【Results】 150 valid questionnaires were collected. The mean of all patients?蒺 correctly answered items was 4.3 ± 1.5. The participants were considered having diet control knowledge if he or she correctly answered ≥6 items and the rate of having knowledge for all patients was 22.0%. Three fourths of patients were well informed about the hazard of seafood, organ meat or alcohol for gout control and 66.0% knew that red-meat will increase serum uric acid (sua). Only 8.7% of patients knew that low-fat dairy products could lower sua, and 40.7% still thought purine-rich vegetables were bad for gout patients, 76.0% thought that soy products could increase sua. There were 14.7%, 12.7% and 40.0% of patients who were aware that fruits rich in fructose, sugar-sweetened soft drinks and fruit juices were unfavorable to gout control, respectively. Logistic regression revealed that gender and acute attack frequency significantly predicted that patients had diet control knowledge. 【Conclusion】 Gout patients?蒺 knowledge of gout-related diet control was out-of-date, especially about some new risk factors and protective factors. Patients’ education in future should be emphasized on the update of gout diet control knowledge, especially for male patients and patients suffered repeated gout attack.  
        关键词：gout;diet control;questionnaires;patients   
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      • Vol. 36, Issue 2, (2015)
        关键词：endoscopic examination;maxillary sinus;dentigerous cyst;frontal recess of tears   
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