最新刊期
卷 41 ， 期 1 ， 2020
- 关键词：innate immunity;antiviral immunity
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发布时间：2023-12-12 -
- 关键词：cellresponse;innate immunity;adaptiveimmune responses;cell death;unfoldedprotein response;autophagy;stressgranule
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发布时间：2023-12-12 - 摘要：Gliomas are the most common primary malignant tumors in the central nervous system，and more than half of them are WHO grade IV glioblastomas. Even if surgery，concurrent chemoradiotherapy and adjuvant chemotherapy were applied，median survival of GBM patients is still only 14.2 months. Immunosuppression is an important feature of malignant glioma. Immunotherapy which reverse immunosuppression may be the most promising way to improve the treatment effect of glioma patients in recent years. This article reviews the recent progress of glioma immunotherapy and the research progress of regulatory T cells（Treg）and glucocorticoid-induced tumor necrosis factor receptor（GITR）in the glioma microenvironment. We hope this article will provides new research ideas for glioma immunotherapy.关键词：glioma;microenvironment;regulatory T cells;GITR
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发布时间：2023-12-12 - 摘要：Sepsis is defined as a life- threatening organ dysfunction caused by a deregulated host response to infection and is the leading cause of mortality in intensive care units. Although the exact pathogenesis of sepsis remains unclear，immunosuppression is considered to play a key role. Sepsis-induced immunosuppression is characterized by a failure to eradicate the primary infection and by development of secondary nosocomial infections， resulting in a prolonged hospitalization and increased death rate in sepsis patients. Early recognition of immunosuppression and appropriate intervention will benefit these patients. This review helps clinicians identify patients with immunosuppression through high-risk population screening and biomarkers detection，and introduces immunostimulants that have been used in clinics and clinical trials.关键词：immunosuppression;immunotherapy
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发布时间：2023-12-12 - 关键词：long non- coding RNA;X inactive specific transcript;bone marrowmesenchymal stem cell;chemokine receptor 4
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发布时间：2023-12-12 - 摘要：【Objective】To investigate the effect of PD-related gene SNCA mutated in A53T on methylation modification in the dopaminergic neurons from the mouse midbrain.【Methods】The midbrain tissue from the A53T mutant human α-synuclein（hA53T α-syn）transgenic mice and non-transgenic（nTg）mice were isolated α-synmice. Bisulfite-sequencing（BS-seq）was utilized for analyzing the DNA methylation of 12-month-old of hA53T α-synmice and nTg mice at a whole genome level. Subsequently，differentially methylated regions（DMRs）were screened for GO enrichment analyses.【Results】Through comparative analyses，481 DMRs were found. Among the data ，hypermethylated and hypomethylated DMRs accounted for 257 and 224 respectively. These DMRs involved in ubiquitin degradation pathway-related genes， including Ubqln2，HECTD4，Rnf157 genes；serine/threonine protein kinase PINK1 gene，etc. Enrichment data revealed that the genes containing DMRs projected to 545 GO sub-terms，and significantly enriched in anatomical structure development，dendrite development，nervous system development，neuronal projection，etc.【Conclusion】The A53T mutation of SNCA gene which is related to PD could introduce DNA methylation alterations in mouse midbrain.
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发布时间：2023-12-12 - 关键词：LncRNA HULC;glioblastoma;proliferation;apoptosis;orthotopic xenotransplantationmodel
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发布时间：2023-12-12 - 摘要：【Objective】The aim of this study is to investigate whether Fuzi polysaccharide（FPS）inhibits calcification of vascular smooth muscle cells（VSMC）and its underlying mechanism involving ceramide signaling.【Methods】We used Ox- LDL to induce in vitro model of human VSMC calcification in this study. FPS at different concentrations was used to treat human VSMC. Cell calcification was assessed by alizarin red staining. The mRNA expressions of osteogenic differentiation markers including Msx2，Osterix and BMP2，and contractile marker SMA were analyzed by qRT- PCR. The protein expressions of Msx2 and BMP2 were analyzed by western blot. Cell apoptosis was examined by TUNEL. Additionally，we investigated the effect of FPS on ceramide levels and N- SMase activity in VSMC. 【Results】We found that FPS inhibits Ox- LDL- induced VSMC apoptosis and calcification. Ceramide participates in Ox- LDL- induced apoptosis and calcification of VSMC. FPS reduces N- SMase activity and ceramide levels in Ox- LDL- treated VSMC. Collectively ， reducing N-SMase activity and ceramide levels could become a promising strategy for the treatment of vascular calcification.【Conclusion】We demonstrate that FPS attenuates VSMC calcification via targeting ceramide signaling.
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发布时间：2023-12-12 - 关键词：uveal melanoma;miR-127-3p;MAPK4;proliferation;migration;invasion
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发布时间：2023-12-12 - 关键词：miRNA-138;JNK/p38 MAPK pathway;cardiomyocyte;apoptosis
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发布时间：2023-12-12 - 关键词：HBV;sleep disorder;investigation
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发布时间：2023-12-12 - 摘要：【Objective】To observe macular choroidal thickness and topographical variation in Chinese healthy and myopic children and to investigate the correlated factors. 【Methods】 A total of 196 myopic children treated at Hainan Province Eye Hospital were selected and divided into hyperopia ，emmetropia，low，moderate and severe myopia groups according to their spherical equivalents（SE）. Axial length（AXL），subfoveal choroidal thickness（SFCT）and 1 mm ，3 mm nasal，temporal，superior，and inferior to the foveal choroidal thickness were recorded. Forty- five students from Tung Wah Group of Hospital′s MA Kam Chan Memorial Primary School in Hong Kong were also recruited in this 1-year longitudinal study. Children were grouped to myopic group and emmetropic or hyperopic group according to SE ，thickness changes of choroid were compared among children with or without myopic shift. The correlation among choroidal SE，and AXL variations were also investigated.【Results】Mean SFCT was 250（204~314）μm. The choroid was thinner at the nasal and inferior sectors ，the thinnest being at 3mm nasal to the fovea ，and the thickest at 1mm temporal to the fovea. Choroidal thickness in all orientations became thinner with progressively descending refractive degree. SFCT and 1 mm around fovea decreased most compared with other surrounding directions in all groups（Kruskal- Wallis test，P < 0.05）. The choroidal thickness in subfovea and other sectors got thinned after 1 year follow-up time（Pair t test，P < 0.05）.The variation of the choroidal thickness in fovea and surrounding positions was positively correlated with the change of SE and negatively related to the change of the AXL. The myopic group had a faster descent of SE and a faster growth of AXL than the emmetropic or hyperopic group. SFCT and surrounding choroidal thickness showed a progressive descent in the myopic group，but a slight decrease in the emmetropic or hyperopic groups.【Conclusions】There is significant topographic variation of choroidal at different regions of the macular. The choroidal thickness decreases faster in myopes. SFCT is more sensitive to myopic progression compared with surrounding regions.关键词：myopia;choroidal thickness;longitudinal study;cross-sectional study
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发布时间：2023-12-12 - 摘要：【Objective】To study the expression of Tip60（KAT5）in Oral squamous cell carcinoma（OSCC），and to investigate the relationship of Tip60（KAT5）and OSCC.【Methods】Forty-nine samples of OSCC and 36 samples of nor⁃ mal tissue adjacent to OSCC were collected and analyzed retrospectively. The expression of Tip60（KAT5）was detected by immunehisto chemistry using Envision detection system. OSCC cell line Cal27 and UM1 cultured in vitro were treated with Nu9056，an inhibitor of Tip60（KAT5）for 24h，respectively. Then the effect of different concentrations and times on the growth of Cal27 and UM1 cells was evaluated with MTT assay.【Results】The expression of Tip60（KAT5）in OSCC was significantly higher than that in normal tissue adjacent to OSCC（P=0.000）. Its expression was significantly positively correlated with the degree of histological differentiation（r=0.461，P=0.001）. With the increase of concentration，the proliferation inhibition rate of Nu9056 on Cal27 and UM1 cells gradually increased，showing a concentration- dependent and time-dependent relationship（P<0.05）.【Conclusions】The expression of Tip60（KAT5）protein in oral squamous cell carcinoma tissues was related to the degree of histological differentiation ，Tip60（KAT5）can be used as oral squamous cell carcinoma progression and prognosis of biological indicators.关键词：oral squamous cell carcinoma;Tip60（KAT5）;Nu9056
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发布时间：2023-12-12 - 关键词：schizophrenia;agitation;ziprasidone;meta-analysis;meta-regression
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发布时间：2023-12-12 - 摘要：【Objective】To investigate the effect of adding different preparations containing luteinizing hormone activity in patients with normal ovarian reserve but suboptimal response during GnRH agonist protocol when undergoing IVF/ICSI- ET.【Methods】872 infertile patients with normal ovarian reserve but suboptimal response to FSH during GnRH agonist protocol were enrolled. According to the supplementation of different preparations containing LH activity ，patients were divided into three groups，including low- dose hCG group（n=28），rLH group（n=319）and HMG group（n=525）. The clinical parameters and pregnancy outcomes were retrospectively compared among three groups.【Results】The proportion of ultra-long GnRH-a protocol in hCG group was higher than that in HMG group（14.3% vs. 1.1%，P＜0.001）. The total duration and dosage gonadotrophin in hCG group were more than that in HMG group ［15.0（13.0~16.8）vs. 13.0（12.0~15.0）days ，P = 0.027 ；2 925（2 531~3 900）vs. 2 550（2 100~3 225）U ，P = 0.046］. The total duration and dosage gonadotrophin in rLH group were less than that in HMG group［13.0（12.0~14.0）vs. 13.0（12.0~15.0）days，P = 0.009；2 400（1 950~3 075）vs. 2 550（2 100~3 225）U ，P = 0.009］. There were 53.6%（15/28）patients who still showed suboptimal response after the administration of HMG or rLH in hCG group. The clinical pregnancy rate（69.2%，58.6% vs.63.8%；P＞0.05）and live birth rate（65.4%，49.6% vs. 53.1%；P＞0.05）were similar among these groups.【Conclusions】 For patients with normal ovarian reserve but suboptimal response during GnRH agonist protocol ，the supplementation of different preparations containing LH activity showed comparable effect on pregnancy outcomes. The addition of low- dose hCG was effective even when patients still showed suboptimal response after the administration of HMG or rLH.关键词：gonadotropin releasing hormone agonist;pituitary down- regulation;suboptimal response;luteinizing hormone
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发布时间：2023-12-12 - 摘要：【Objective】To investigate the expression of FosB in invasive breast cancer and its correlation to the ultrasound findings and clinical significance.【Methods】Immunohistochemical and ultrasound examination were performed for 155 cases with invasive breast cancer，36 cases with paracancerous tissue and 30 cases with normal breast tissue. To investigate the correlations between the different expression of FosB with histological grade ，lymph nodes，and ultrasound findings.【Results】①The expression of FosB in paracancerous tissue and normal breast tissue was higher than that in invasive breast cancer（P＜0.01）. ②Based on immunohistochemical staining，high positive expression rate of FosB was associated with low histological grade，ER positive and PR positive（P＜0.05）. There was no correlation between FosB and age，tumor diameter，lymph nodes，Her2 and clinical stages（P＞0.05）③The expression of FosB was higher in microcalcification positive and lymph nodes bigger tissues（P＜0.05）. There was no correlation between FosB and tumor size， regular shape，boundary，posterior attenuation ratio and blood flow（P＞0.05）.【Conclusion】The expression of FosB in invasive breast cancer was lower than that in paracancerous tissue and normal breast tissue. The different expression of FosB was correlated with histological grade，ER positive and PR positive. To study the expression of FosB in invasive breast cancer may be helpful for differential diagnosis and targeted treatment. The different expression of FosB was correlated with microcalcification，lymph nodes，and combined FosB with ultrasound findings may contribute to prognostic evaluation of breast cancer.关键词：FosB;invasive breast cancer;ultrasound;immunohistochemical
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发布时间：2023-12-12 - 摘要：【Objective】To prepare rapamycin（RAPA）sustained-release film and to evaluate its dissolution.【Methods】RAPA sustained- release film was created by using polymer polyactioglyconic acid （PLGA），copolymer of polyactic acid（PLA）and polyglycolic acid（PGA）. Drug content of the sustained-release film was determined using specificity test，recovery，relative standard deviation（RSD）and stability test. Then，the dissolution of the sustained- release film was analyzed.【Results】The concentration of RAPA had a linear relationship with peak area，which ranged between 0.408 μg/mL and 40.8 μg/mL through the standard curve. The specificity test of the drug content determination indicated the excipient of the film and the solution with 0.3% sodium dodecyl sulfate（SDS）did not affect in determining the RAPA content. The recovery and RSD were excellent through drug content determination in blank films，which had three different levels of RAPA concentrations. The mean RAPA content of the sustained-release films was（112.6±10.1）μg（RSD 8.99%）through the drug content determination of the films，and the stability of RAPA with 0.3% SDS was good within 15 days. In addition，dissolution test of the sustained- release film indicated that the amount of drug release reached a high level and sustained up to 15 days.【Conclusion】 The RAPA sustained-release film with certain behavioral characteristic parameters had a stable drug content and favorable sustained-release property，and it may have certain application potential in anti-proliferation after glaucoma filtering surgery.关键词：rapamycin;sustained-release drug;dissolution;glaucoma;anti-proliferation
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发布时间：2023-12-12 - 摘要：【Objective】 Cloud point extraction （CPE） combined with hydrophilic interaction （HILIC） was employed to simultaneously determine the anti-AIDS drugs in the serum of rats. 【Methods】Triton X-114 was used as extraction medium to extract four nucleoside antiviral drugs from rat serum. Response surface methodology was employed to further optimize CPE parameters. The content of four anti-AIDS drugs in rat serum was simultaneously determined by HILIC method.【Results】 The optimized conditions were as follows：5%（w/v）Triton X- 114，0.3 mol/L NaCl，PH 5.0， the water- bath equilibrium 20 min at 40 ℃ . Under optimized extraction conditions，the extraction rates of the four anti- AIDS drugs were all over 85.0%，which was pretty close to the predicted result. The extracted samples were analyzed under the optimal chromatographic conditions. The recovery rate was over 75.0% and RSD was less than 5.0%. The optimized chromatographic conditions were as follows：Ze month- CN（250 × 4.6 mm，5 micron）as stationary phase，mobile phase （methanol：acetonitrile：ammonium acetate buffer）（5∶5∶90），column temperature 35 ℃，detection wavelength 275 nm，flow rate 0.5 mL/min.【Conclusion】The developed method of CPE combined with HILIC can enrich four anti-AIDS drugs in rat serum ，which is simple，environment- friendly and can be used to accurately determine the blood concentration of four anti-AIDS drugs in rat serum，providing a new method for clinical blood concentration monitoring.关键词：hydrophilic interaction chromatography;anti-AIDSdrugs;serum
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发布时间：2023-12-12
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