【Objective】To study the resistance reversion of rapamycin on ovarian cancer cell line SKOV3/DDP，and ex? plore its underlying molecular mechanisms.【Methods】MTT method was used to detect the cell toxicity，drug-resistant multi? ple and reversing multiple of cisplatin-resistant ovarian cancer cell line SKOV3/DDP；Western blot was used to detect the changes of Akt/mTOR Pathway induced by rapamycin.【Results】① MTT detected that when rapamycin concentration was 25，50，100，500 and 1 000 μg/L，its inhibition rates on cisplatin-resistant ovarian cancer cell line SKOV3/DDP were 4.48%，25.30%，35.86%，67.82%，81.43%. The concentration of 25 μg/L was selected to be the reversal concentration，be? cause its maximum rate was less than 5%. ② The resistant index（RI）of cisplatin-resistant ovarian cancer cell line SKOV3/ DDP was 2.21. ③ The reversal fold of 25 μg/L rapamycin on cisplatin-resistant ovarian cancer cell line SKOV3/DDP was 1.63. ④ Western blot results：After the addition of rapamycin，expression of p-mTOR and its downstream protein p-p70s6k in SKOV3 and SKOV3/DDP was significantly reduced. Meanwhile，there was a feedback increase in p-Akt.【Conclusions】 Rapamycin has a reversal effect on cisplatin-resistant ovarian cancer cell line SKOV3/DDP. Its reversal mechanism may be inhibiting the cell proliferation and promoting cell apoptosis by depressing the expression of p-mTOR and its downstream pro? tein p-p70s6k in Akt/mTOR Pathway.