Abstract:【Objective】 To examine the effect of vitrification on morphology and apoptosis in human ovarian cortex tissue. 【Methods】 Ovarian tissues came from 4 cases of partial ovariectomy

with necessity of diagnosis or treatment. In each case

the cortex was cut into small pieces. After that they were randomly divided into Fresh Group and Vitrification Group. The morphology of follicles and stromal cells were examined by HE staining

and the cell apoptosis in situ was analyzed by TUNEL assay. Furthermore

we quantified the expression of Cleaved Caspase-3 (an indicator of apoptosis). 【Results】 The percentage of primordial follicles

primary follicles and secondary follicles in Fresh Group are (55.5 ± 4.9)%

(34.6 ± 1.6)%

and (9.9 ± 7.6)%

respectively

while the percentage in Vitrification Group are (51.2 ± 5.1)%

(36.7 ± 1.6)%

and (12.1 ± 3.5)%. The percentage of primordial follicles

primary follicles and secondary follicles and the apoptosis in situ show no significance between these two groups. Besides

the expression of Cleaved Caspase-3 in ovarian tissue is the same between two groups. 【Conclusion】 The strategy of vitrification of human ovarian tissue is viable

and still needs more researches.