【Objective】We aimed to explore the diagnostic value of DNA fragmentation index（DFI）and acrosin activi? ty in male infertility.【Methods】Nine hundred and two semen samples were collected from patients and assessed for the DNA fragmentation index by sperm chromatin dispersion test，acrosin activity，as well as standard sperm parameters according to the WHO criteria. Statistical analysis was performed with SPSS.【Results】Statistically significant differences were observed in age，sperm concentration，total motility，progressive rate and acrosin activity among different group of sperm DNA dam? age（≤10%，11~20%，21~30%，≥31%）. Sperm acrosin activity also showed difference in sperm concentration，total num? ber，total motility，progressive rate，normal morphology rate，teratozoospermia index（TZI）and sperm DFI. The DNA frag? mentation rate and sperm concentration，sperm motility，forward motility rate，total sperm count and acrosin activity was neg? atively correlated，while it is negative correlatd with TZI. Acrosin activity and sperm concentration，sperm motility，forward motility rate，sperm count and normal morphology rate was positively correlated，while it is negative correlated with the ab? normal rate of head，sperm deformity index，DNA fragmentation rate.【Conclusion】Sperm DNA damage and acrosin activity could partly reflect the quality of sperm. Moreover，sperm DFI maypredict the sperm motility part while the acrosin activity more likely related to sperm morphology.