Abstract:【Objective】 The aim of this study was to investigate the effect of scavenger receptor class B type I (SR-BI) overexpression on the uptake of high density lipoprotein (HDL) and provide a novel thought to research the role of SR-B1 in reverse cholesterol transport (RCT) and atherosclerosis (AS). 【Methods】 We adopted recombinant vectors rAAV2/1-SR-BI-IRES-EGFPtotransfect human HepG2 hepatoma cells. Then

the expressions of SR-BI mRNA and protein were detected by Real-time RT-PCR analysis and Western blot analysis after transfection. HDL was labeled by radioiodination of the protein component using 125I and radioactivity were measured after transfection. 【Results】 Compared with the control and rAAV2/1-IRES-EGFP transfection group

the expressions of SR-BI mRNA and protein were significantly increased as well as the increasing uptake of 125I-HDL (by 1.28 and 1.40 times

respectively)after rAAV2/1-SR-BI-IRES-EGFP transfection (P < 0.05). RAAV2/1-SR-BI-IRES-EGFP was found distributed both in the cytoplasm and cell membrane in serum-starved cells. However

HDL resulted in a marked redistribution of the bulk of rAAV2/1-SR-BI-IRES-EGFP to the cell surface. 【Conclusion】 SR-B1 overexpression increases the uptake of HDL in HepG2 hepatoma cells

which might occur in the cell surface. Thus

the overexpression of SR-BI gene could increase the RCT pathway by promoting cholesterol ester uptake from plasma HDL.