Abstract: 【Objective】 This study aims to investigate the in vitro effects of ticagrelor on Ag endocytosis and presentation by dentritic cells (DC)

thereby exploring the role of ticagrelor in immune regulation. 【Methods】 P2Y12 expression in DC was analyzed by RT -PCR and Western -blotting. DC were divided into normal culture group

simple ADPβS group and ticagrelor + ADPβS group. Fluorescein isothiocyanate (FITC) -Dextran was used to test DC endocytosis. Ovalbumin (OVA) -specific T (OT -2) cells were purified from the spleen. The carboxyl fluorescein succinimidyl ester(CFSE) -labeled T cells were co -cultured with OVA -loaded DC for 4 d

and T cell proliferation was analyzed by flow cytometry. 【Results】 RT -PCR and Western blot analysis revealed expression of functional P2Y12 receptors in murine DC. Ticagrelor blocked ADPβS -induced DC endocytosis

and significantly diminished the ability of DC to simulate Ag -specific T cells. 【Conclusion】 Our study demonstrates that antiplatelet drug ticagrelor directly inhibits DC function via P2Y12 receptors

which is probably beneficial for treatment of coronary heart disease.