【Objective】 To investigate the effects and the underlying mechanism of prostaglandin E2 (PGE2) on the proliferation of human CD34+ cells. 【Methods】 Human CD34+ cells were isolated by MACS microbead kits

and the purity was identified by flow cytometry. Then 5×103 /well human CD34+ cells were treated with different concentration dmPGE2. Two hours after cells exposure to ice

erythroid burst forming unit (BFU-E) number and granulocyte monocyte colony forming unit (CFU-GM) number were evaluated by colony-forming assay. Furthermore

cell cycle distribution was analyzed by flow cytometry

and the expression of survivin mRNA

β-catenin and survivin protein of human CD34+ cells was detected by QRT-PCR and Western blot

respectively. 【Results】 The percentage of CD34+ cells in the whole isolated cells was up to 95%. The number of BFU-E and CFU-GM in the 1 μmol/L dmPGE2-treated group was higher than that in the control group and that in the other treatment group (P < 0.05). The proportion of human CD34+ cells which entered into S/G2M phase was 2.18 times of the control group (P < 0.05). The expression level of survivin mRNA

survivin protein andβ-catenin protein was also elevated significantly after exposure to 1 μmol/L dmPGE2. 【Conclusion】 PGE2 promotes human CD34+ cells proliferation. The underlying mechanism was involved in more quiescent CD34+ cells entering into cell division cycle

which was mediated by the elevated survivin and β-catenin after exposure to dmPGE2.