大鼠自体原位肝移植肺组织TNF-α?IL-1β和IL-6的表达

罗刚健; 池信锦; 张瑷兰; 朱国松; 黑子清

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大鼠自体原位肝移植肺组织TNF-α?IL-1β和IL-6的表达

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- 大鼠自体原位肝移植肺组织TNF-α?IL-1β和IL-6的表达
- TNF-α, IL-1β, and IL-6 Genes Transcription and Expression Level of Lung Tissue in Rats Experienced Orthotopic Autologus Liver Transplantation
- 中山大学学报（医学科学版） 2012年33卷第4期
- 作者机构：
  
  (中山大学附属第三医院麻醉科, 广州 ),广东,510630
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 网络首发：2012-07-20，
  
  纸质出版：2012
- 稿件说明：
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罗刚健, 池信锦, 张瑷兰, 等. 大鼠自体原位肝移植肺组织TNF-α?IL-1β和IL-6的表达[J]. 中山大学学报（医学科学版）, 2012,33(4).

LUO Gang-jian, CHI Xin-jin, ZHANG Ai-lan, et al. TNF-α, IL-1β, and IL-6 Genes Transcription and Expression Level of Lung Tissue in Rats Experienced Orthotopic Autologus Liver Transplantation[J]. Journal of Sun Yat-sen University (Medical Sciences), 2012, 33(4).
罗刚健, 池信锦, 张瑷兰, 等. 大鼠自体原位肝移植肺组织TNF-α?IL-1β和IL-6的表达[J]. 中山大学学报（医学科学版）, 2012,33(4). DOI：

LUO Gang-jian, CHI Xin-jin, ZHANG Ai-lan, et al. TNF-α, IL-1β, and IL-6 Genes Transcription and Expression Level of Lung Tissue in Rats Experienced Orthotopic Autologus Liver Transplantation[J]. Journal of Sun Yat-sen University (Medical Sciences), 2012, 33(4). DOI：

摘要

【目的】观察大鼠自体原位肝移植围术期肺组织TNF-α、IL-1β 和IL-6 基因表达和水平的变化.方法】选取健康SPF 级SD大鼠30只

随机分为假手术对照组(S组

n=6)和移植(肝脏再灌注)后4、8、16、24 h组(M4、M8、M16和M24 组

每组n = 6).肺组织病理损伤

RT-PCR检测肺组织TNF-α、IL-1β和IL-6基因表达

ELISA分析肺组织匀浆TNF-α、IL-1β和IL-6水平.【结果】 ①病理学检查: 假手术组肺组织结构基本正常

移植组表现为肺间质明显出血、血

中性粒细胞浸润显著增多

以肝脏再灌注后8 h 最为明显.与S组相比

M4、M8、M16、M24组肺含水率显著升高

TNF-α、IL-1β及IL-6的基因表达均显著上调(P < 0.05)

在肝脏再灌注8h达到峰值.S组相比

M4、M8、M16、M24组肺组织匀浆TNF-α、IL-1β及IL-6水平显著升高(P < 0.05);TNF-α、IL-1β在肝脏再灌注8h达到峰值

而IL-6则在肝脏再灌注后16 h达到峰值? 【结论】大鼠自体原位肝移植围术期肺组织TNF-α、IL-1β和IL-6浓度显著升高

基因表达水平增高

可能与围术期发生急性肺损伤有关.

Abstract

【Objective】 To investigate the gene transcription and expression level of TNF-α

IL-1β

and IL-6 in lung tissue of rats experienced orthotopic autologus liver transplantation. 【Method】 Thirty healthy SPF Sprague-Dawley rats were randomly divided into five groups. According to the different times after liver reperfusion

the rats were divided into sham group (S

n = 6)

4 h after liver reperfusion group (M4

n = 6)

8 h after liver reperfusion group (M8

n=6)

16 h after liver reperfusion group (M16

n=6)

24 h after liver reperfusion group (M24

n = 6). Lung tissues of the each animal in different groups were collected and sent to analyze the morphology change as well as he genes transcription and expression level of TNF-α

IL-1β

and IL-6. RT-PCR was adopted to evaluate the TNF-α

IL-1β

and IL-6 genes transcription level and ELISA method was performed to determine the TNF-α

IL-1β and IL-6 genes expression level in lung tissue. 【Results】 (1) Lung tissue histological analysis shown the rats in sham group exhibited the normal structure of the lung tissue and seldom inflammatory cells infiltration

while the rats received orthotopic autologus liver transplantation revealed collapse of the pulmonary structure

severely infiltration of inflammatory cells into the intra-alveolar and interstitial spaces as well as edematous interstitial space. (2) Comparing with sham group

lung water content and genes transcription level of TNF-α

IL-1β and IL-6 in M4

M16 and M24 groups significantly increased (P < 0.05)

while each of them reached the peak value at 8h after liver reperfusion. (3) Comparing with sham group

genes expression level of TNF-α

IL-1β and IL-6 in M4

M16 and M24 groups significantly increased (P < 0.05)

TNF-α and IL-1β approached the top value at 8 h after liver reperfusion

while IL-6 reached the peak value at 16 h after liver reperfusion. 【Conclusion】 The genes transcription and expression level of TNF-α

IL-1β and IL-6 in lung tissue of the rats experienced orthotopic autologus liver transplantation were significantly up-regulated

which may contribute to the acute lung injury after liver transplantation

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