【Objective】To investigate the correlation of human epidermal growth factor receptor 2（HER2）with the clinicopathologic features and molecular subtypes of urothelial bladder cancer（UBC）.【Methods】A total of 70 paraffin- embedded UBC samples，50 invasive and 20 non-invasive，were examined for the HER2 protein overexpression by immu⁃ nohistochemistry（IHC）and for the gene amplification by fluorescent in situ hybridization（FISH）. IHC was used to detect the luminal markers（CK20，GATA3），basal markers（CK14，CK5/6）and Ki67 protein expression for 50 cases of invasive UBC. Pearson′s chi-square test and Fisher′s exact test were performed to analyze the association between HER2 and clinicopathological characteristics such as gender，age，pathohistological diagnosis，histologic grade，Ki67，molecu⁃ lar subtypes，tumor maximum diameter，muscular invasion，lymphovascular invasion，lymph node metastasis and clini⁃ cal stage. Statistical significance was set at P < 0.05.【Results】The positive rate of HER2 in invasive UBC was 44%（22/ 50）and no HER2 positive was found in non-invasive UBC. In 50 invasive UBC cases，the HER2 positive rate was significantly higher in the luminal subtype than that in the basal（P = 0.003）. HER2 positive was significantly correlated with high grade（P = 0.006），Ki67≥30%（P = 0.001）and lymphovascular invasion（P = 0.004）；but unrelated to muscle invasion，lymph node metastasis，clinical stage，tumor maximum diameter，gender and age（all P > 0.05）. 【 Conclusions 】HER2 is closely related to the aggressiveness of invasive high- grade UBC. HER2 status combined with molecular subtypes may help to screen potential UBC patients for whom HER2 targeted therapy is effective.