Abstract: 【Objective】 To study the anti-tumor effect by targeting β2-adrenoceptor in osteosarcoma and the underlying molecular mechanism. 【Methods】 MTT assay was used to detect the anti-proliferation effect in osteosarcoma following the cells were treated with a β2-adrenoceptor antagonist ICI118

551.Then

flow cytometry was employed to detect the cell cycle arrest and apoptosis. Finally

the concerning signal pathway wastested by Western blot. 【Result】 Targeting β2-adrenoceptor could inhibit osteosarcoma cell proliferation. Significant G0/G1 cell cycle arrest and apoptosis were detected through flow cytometry following ICI118

551 incubation. Furthermore

the protein level of p-ERK was found down-regulated remarkably. 【Conclusion】 Together

targeting β2-adrenoceptor possesses potent anti-tumor effect in osteosarcoma cells. The ERK signaling pathway probably play a critical role in this process.