Abstract: 【Objective】 To investigate endometrium heparin binding epithelial growth factor (HBEGF) and leukemia inhibitory factor (LIF) expressioninduced by different stage of mouse embryos .【Methods】 The embryos transferred to pseudopregnant mouse were divided into 6 groups: ①8 cell embryo (8c)

②8 cell embryo + 4 cell embryo(8c+4c)

③8 cell embryo + 2 cell embryo(8c+2c)

④4cell embryo(4c)

⑤2cell embryo(2c)

⑥5μLmedium(normal control

NC). Fluorescent quantitation PCR

immunohistochemistry

and Western blot analysis were performed to detect HBEGF and LIF expression levels in the mice endometrium. To observe the implantation numbers after 7 days of embryo transfer. 【Results】 The mRNA and protein expression level of HBEGF and LIF had significant diffidence among groups(all P < 0.001). The expression of HBEGF and LIF had no significant diffidence between group 8c+4c and 8c+2c (P > 0.05)

but had significant diffidence compared with group 8c (P < 0.05). The expression of HBEGF and LIF had no significant diffidence between group 4c and 2c (P > 0.05)

but had significant diffidence compared with group NC (P < 0.001).The implantation number of group 8c+4c and 8c+2c had an upward tendency compared with group 8c. 【Conclusion】 Poor quality embryo could induce endometrium receptivity factors HBEGF and LIF expression. The inducing effects had no significant diffidence between different poor stage embryos. The inducing effects of poor embryo were lower than good embryo.