华南农业大学兽医学院,广东 广州 510642
吕进微,第一作者,研究方向:人兽共患寄生线虫的寄生适应性机制,E-mail:13984152960@163.com
收稿:2025-08-04,
修回:2025-12-08,
录用:2025-12-15,
纸质出版:2026-01-20
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吕进微,林玉,冯耀宇等.应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染[J].中山大学学报(医学科学版),2026,47(01):11-16.
LÜ Jinwei,LIN Yu,FENG Yaoyu,et al.Identification of Angiostrongylus cantonensis in Wild Rodent Feces via cytb Sequences[J].Journal of Sun Yat-sen University(Medical Sciences),2026,47(01):11-16.
吕进微,林玉,冯耀宇等.应用cytb序列鉴定野鼠粪便中的广州管圆线虫感染[J].中山大学学报(医学科学版),2026,47(01):11-16. DOI: 10.11714/jsysu.med.YX20250099.
LÜ Jinwei,LIN Yu,FENG Yaoyu,et al.Identification of Angiostrongylus cantonensis in Wild Rodent Feces via cytb Sequences[J].Journal of Sun Yat-sen University(Medical Sciences),2026,47(01):11-16. DOI: 10.11714/jsysu.med.YX20250099.
目的
2
应用多种分子标记,评估其在鉴定广州市花都区花山镇野鼠粪便中的广州管圆线虫的有效性,并分析野鼠的感染状况。
方法
2
捕获广州花都区花山镇128只野鼠,采集野鼠粪便,采用FastDNA SPIN Kit for Soil试剂盒提取粪便总DNA,通过PCR扩增获得
cytb
、
cox I
和ITS2片段,PCR产物经测序鉴定,并对测序产物进行系统发育分析。
结果
2
通过PCR扩增和测序鉴定,仅有5份样本扩增出的
cytb
序列没有套峰,可以准确鉴定为广州管圆线虫。结果表明该地区褐家鼠的广州管圆线虫感染率为7.58%(5/66),而黄胸鼠、黄毛鼠、臭鼩鼱及板齿鼠中未检测到
cytb
序列,表明褐家鼠是广州管圆线虫的适
宜宿主,且
cytb
序列适用于从野鼠粪便中鉴定广州管圆线虫的感染情况。
结论
2
Cytb
序列是鉴定野鼠粪便中广州管圆线虫的良好分子标记,可以用于长期监测野鼠中的广州管圆线虫流行情况和危害潜力。
Objective
2
Several molecular markers were evaluated for their reliability in detecting
Angiostrongylus cantonensis
in wild rodent feces and for determining the infection status of these rodents.
Methods
2
A total of 128 wild rodents were captured in Huashan Town, Huadu district, Guangzhou. Their fecal samples were collected and total DNA was extracted with the FastDNA SPIN Kit for Soil. PCR amplification was performed to obtain
cytb
,
cox I
and ITS2 gene fragments, followed by Sanger sequencing and phylogenetical analysis.
Results
2
Among all 128 fecal samples, only 5 had clean PCR bands and unambiguous
cytb
sequences suitable for reliable species identification. The infection rate of
A. cantonensis
was 7.58% (5/66) in
Rattus norvegicus
, whereas no
cytb
fragments were detected in
Rattus tanezumi
,
Rattus losea
,
Suncus murinus
and
Bandicota indica
. These data indicate that
R. norvegicus
is a highly suitable host for
A. cantonensis
, and the
cytb
gene serves as an effective molecular marker for detecting
A. cantonensis
in wild rodent feces.
Conclusion
2
The
cytb
is an effective molecular marker for detecting
A. cantonensis
in wild rodent feces, enabling long-term surveillance of its prevalence and potential public health threat.
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